Heat-Loving Bacteria

The human body maintains a body temperature of around 37°C, the optimal temperature for the various functions it must perform. If our body temperature becomes too high, the various mechanisms that make our bodies run break down, and it becomes impossible to stay alive. However, there are some organisms found on the Earth that live in extremely hot places, like the places where hot water comes out of the earth to form hot springs. Many of these organisms are bacteria, and some live in hot water that is upwards of 80-100°C. These bacteria, referred to as “thermophiles” and “ultra-thermophiles,” are able to maintain the activities necessary to sustain life at these high temperatures. They are able to live in such environments because their bodies make heat-resistant proteins.

Defense/Stability:: Heat resistance

Coexistence with nature

Saiki et al. Science, Jan 29;239(4839):487-91, 1988. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

In order to artificially replicate DNA, it is necessary to first heat it to 94°C in order to break its double helix structure into individual strands. In 1988, Kary Mullis, a researcher working for Cetus Corporation, came up with the PCR method, which uses the heat resistant DNA polymerase enzymes present in thermophilic bacteria to copy DNA sequences. Science, an academic journal, featured a paper describing this idea, which allows people to easily copy and amplify DNA using this technique. By studying how thermophiles’ bodies work, it may be possible to develop vaccines and medicines that are similarly resilient to high temperatures.

Query	Affected	Num. rows	Took (ms)	Actions
SHOW FULL COLUMNS FROM `contents`	16	16	2	maybe slow
SELECT CHARACTER_SET_NAME FROM INFORMATION_SCHEMA.COLLATIONS WHERE COLLATION_NAME= 'utf8_general_ci';	1	1	13	maybe slow
SHOW FULL COLUMNS FROM `users`	10	10	1	maybe slow
SHOW FULL COLUMNS FROM `groups`	4	4	9	maybe slow
SHOW FULL COLUMNS FROM `permissions`	5	5	1	maybe slow
SHOW FULL COLUMNS FROM `groups_permissions`	3	3	1	maybe slow
SHOW FULL COLUMNS FROM `groups_users`	3	3	1	maybe slow
SHOW FULL COLUMNS FROM `contents_searches`	6	6	9	maybe slow
SHOW FULL COLUMNS FROM `attachments`	11	11	1	maybe slow
SHOW FULL COLUMNS FROM `attachments_revs`	13	13	1	maybe slow
SHOW FULL COLUMNS FROM `contents_related_items`	5	5	1	maybe slow
SHOW FULL COLUMNS FROM `related_items`	6	6	1	maybe slow
SHOW FULL COLUMNS FROM `contents_related_items_revs`	8	8	1	maybe slow
SHOW FULL COLUMNS FROM `contents_tags`	5	5	1	maybe slow
SHOW FULL COLUMNS FROM `tags`	7	7	1	maybe slow
SHOW FULL COLUMNS FROM `tag_groups`	6	6	1	maybe slow
SHOW FULL COLUMNS FROM `contents_tags_revs`	7	7	1	maybe slow
SHOW FULL COLUMNS FROM `contents_revs`	18	18	2	maybe slow
SELECT `Content`.`id`, `Content`.`user_id`, `Content`.`last_edit_user_id`, `Content`.`post_date`, `Content`.`post_date_year`, `Content`.`post_date_month`, `Content`.`post_date_day`, `Content`.`title`, `Content`.`title_en`, `Content`.`content`, `Content`.`plain_content`, `Content`.`page_view`, `Content`.`flag`, `Content`.`mod_count`, `Content`.`created`, `Content`.`modified` FROM `contents` AS `Content` WHERE `id` = 165 LIMIT 1	1	1	0
SELECT `Attachment`.`file_name`, `Attachment`.`dir`, `Attachment`.`model`, `Attachment`.`foreign_key` FROM `attachments` AS `Attachment` WHERE `file_name` <> 'default.jpg' AND `Attachment`.`foreign_key` = (165)	1	1	0
SELECT `ContentsRelatedItem`.`content`, `ContentsRelatedItem`.`related_item_id`, `ContentsRelatedItem`.`content_id` FROM `contents_related_items` AS `ContentsRelatedItem` WHERE `ContentsRelatedItem`.`content_id` = (165)	11	11	0
SELECT `RelatedItem`.`code`, `RelatedItem`.`name` FROM `related_items` AS `RelatedItem` WHERE `RelatedItem`.`id` = 1	1	1	8	maybe slow
SELECT `RelatedItem`.`code`, `RelatedItem`.`name` FROM `related_items` AS `RelatedItem` WHERE `RelatedItem`.`id` = 2	1	1	0
SELECT `RelatedItem`.`code`, `RelatedItem`.`name` FROM `related_items` AS `RelatedItem` WHERE `RelatedItem`.`id` = 4	1	1	0
SELECT `RelatedItem`.`code`, `RelatedItem`.`name` FROM `related_items` AS `RelatedItem` WHERE `RelatedItem`.`id` = 6	1	1	0
SELECT `RelatedItem`.`code`, `RelatedItem`.`name` FROM `related_items` AS `RelatedItem` WHERE `RelatedItem`.`id` = 7	1	1	0
SELECT `RelatedItem`.`code`, `RelatedItem`.`name` FROM `related_items` AS `RelatedItem` WHERE `RelatedItem`.`id` = 8	1	1	0
SELECT `RelatedItem`.`code`, `RelatedItem`.`name` FROM `related_items` AS `RelatedItem` WHERE `RelatedItem`.`id` = 9	1	1	0
SELECT `RelatedItem`.`code`, `RelatedItem`.`name` FROM `related_items` AS `RelatedItem` WHERE `RelatedItem`.`id` = 9	1	1	0
SELECT `RelatedItem`.`code`, `RelatedItem`.`name` FROM `related_items` AS `RelatedItem` WHERE `RelatedItem`.`id` = 9	1	1	0
SELECT `RelatedItem`.`code`, `RelatedItem`.`name` FROM `related_items` AS `RelatedItem` WHERE `RelatedItem`.`id` = 11	1	1	0
SELECT `RelatedItem`.`code`, `RelatedItem`.`name` FROM `related_items` AS `RelatedItem` WHERE `RelatedItem`.`id` = 12	1	1	0
SELECT `ContentsTag`.`id`, `ContentsTag`.`tag_group_id`, `ContentsTag`.`mod_count`, `ContentsTag`.`tag_id`, `ContentsTag`.`content_id` FROM `contents_tags` AS `ContentsTag` WHERE `ContentsTag`.`content_id` = (165)	2	2	0
SELECT `Tag`.`name`, `Tag`.`comment`, `Tag`.`tag_group_id` FROM `tags` AS `Tag` WHERE `Tag`.`id` = 70	1	1	0
SELECT `TagGroup`.`code`, `TagGroup`.`name` FROM `tag_groups` AS `TagGroup` WHERE `TagGroup`.`id` = 6	1	1	0
SELECT `Tag`.`name`, `Tag`.`comment`, `Tag`.`tag_group_id` FROM `tags` AS `Tag` WHERE `Tag`.`id` = 100	1	1	0
SELECT `TagGroup`.`code`, `TagGroup`.`name` FROM `tag_groups` AS `TagGroup` WHERE `TagGroup`.`id` = 7	1	1	0
SELECT `RelatedItem`.`id`, `RelatedItem`.`code`, `RelatedItem`.`name`, `RelatedItem`.`created`, `RelatedItem`.`modified`, `RelatedItem`.`flag` FROM `related_items` AS `RelatedItem` WHERE `code` = 'function' LIMIT 1	1	1	0
SELECT `Content`.`id`, `Content`.`title`, `Content`.`title_en` FROM `contents` AS `Content` WHERE id = (NULL) ORDER BY `id` desc	0	0	0

Message	Memory use
Component intitailization	6.79 MB
Controller action start	6.83 MB
Controller render start	6.90 MB
View render complete	7.92 MB

Message	Time in ms	Graph
Core Processing (Derived)	301.84
Component initialization and startup	16.56
Controller action	38.08
Render Controller Action	65.07
» Rendering View	53.65
» » Rendering APP/views/contents/view.ctp	33.85
» » Rendering APP/views/layouts/default.ctp	17.98
» » » Rendering APP/views/elements/header.ctp	1.52
» » » Rendering APP/views/elements/content_sidebar.ctp	0.32
» » » Rendering APP/views/elements/footer.ctp	0.41

Heat-Loving Bacteria

default

Query Explain:

Memory

Timers

Heat-Loving Bacteria

Request History

Session

Request

Cake Params

$_GET

Cookie

Current Route

Sql Logs

default

Query Explain:

Memory

Timers

Logs

View Variables